| 摘要 |
Apparatus for the continuous filtration of cell cultures, said apparatus being adapted for convenient manual assembly - disassembly, comprises (A) a hollow vessel (10) having an opening (13) at the top,… (B) a stationary, hollow shaft (26) positioned within said vessel (10) in fluid communication with said top opening (13) of said vessel (10) and supported downwardly therefrom without any lower bearing,… (C) a stationary filter unit (35) supported downwardly from said shaft (26) without any lower bearing and without any moving seals between the filter unit or any moving filter support means which could contact the cell suspension, said filter having… (1) a fluid collection cavity in direct communication with the interior of said shaft (26) and the top of said vessel, and… (2) a porous outer surface having a pore size smaller than the cells to be cultured or the carrier particles upon which said cells are attached but sufficiently large to permit permeation of fluid into said fluid collection cavity,… (D) a rotatable agitator means (25) supported downwardly from said shaft (26) and concentrically disposed about said filter unit (35) for rotation by suspension from bearing means (39), (40) having low-friction engagement with said hollow shaft (26),… (E) said shaft (26) having a boss (77) positioned between said filter unit (35) and said bearing means (39), (40) and a spring seat (76) adaptable for holding a spring (75) positioned between said boss (77) and said filter unit,… (F) said agitator means (25) having suspended thereon a plurality of flexible vanes (30) which are substantially equidistantly spaced apart circumferentially and which extend substantially throughout the length of said filter unit (35), said vanes (30) being held between a pair of upper clamp plates (51, 61) and a pair of lower clamp plates (52, 62) with the lower member (51) of the upper pair and the upper member (52) of the lower pair of said clamp plates having side slits circumferentially spaced apart to receive the widths of said vanes (30), and said vanes (30) having elastomeric means at each end compressed between the corresponding pair of said clamp plates. |
