| 摘要 |
Liquified vitreous gel, prepared by a non-extractive technique, has been found to contain a cell proliferation inhibitor whose molecular size is greater than 10,000 daltons. Vitreous isolated from both bovine and chick embryo sources has been found to contain such an activity, which inhibits the growth of endothelial cells prepared from calf aorta. Culture medium conditioned by exposure to calf vitreous hyalocytes (cells found on the periphery of the vitreous gel), is also a source of the high molecular weight inhibitor. The high molecular weight inhibitor is prepared by chromatography of vitreous or hyalocyte-conditioned medium on a column of Bio Gel P-10 or by ultrafiltration. Mateial appearing in the void volume of Bio Gel P-10 (the material whose molecular size is too large to allow it to enter the gel) has a molecular size greater than 13,000 daltons. Ultrafiltration is carried out using a filter whose molecular size cut-off point is 10,000 daltons, therefore material which is retained by the filter has a molecular size of 10,000 daltons and above.
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