| 摘要 |
PURPOSE:To produce mevalonic acid in high efficiency, by culturing a specific microbial strain in a medium added with an organic nitrogen as a sole nitrogen source keeping the carbon source concentration in the culture liquid at a definite level. CONSTITUTION:A microbial strain capable of producing mevalonic acid (e.g. Saccharomycopsis fibuligera IFO 0107, etc.) is cultured in a medium composed of 5-15% carbon source, 0.5-3% organic nitrogen source consisting of peptone, yeast extract, meat extract, casein or soybean flour, 0.01-5% each of inorganic salts such as phosphoric acid salt, potassium salt, magnesium salt and calcium salt and 0.02-0.1% nonionic surfactant and cultured at 20-40 deg.C, preferably 25-35 deg.C under shaking or aeration and agitation. When the pH of the medium reaches >=7 or the dissolved oxygen content of the cultured product passes through the inflection point of the content vs. culture time curve, the substrate is added to the cultured product and the culture is continued while maintaining the carbon source concentration in the cultured liquid to 2-15%, preferably 5-10% to produce the objective mevalonic acid. |
