| 摘要 |
<p>The present invention relates to methods of assay of target analyte binding pair members, and to the reagents useful for carrying out such assays. More specifically, the invention relates to ligand binding or nucleic acid assays using a reagent that comprises a signal moiety, and a moiety complementary to or homologous with all or a portion of the target analyte binding members. Under the appropriate conditions, the signal moiety produces a detectable moiety which can be transferred to a gaseous phase for detection. To serve this purpose, the signal moiety is a moiety capable of reacting with an effective second agent to produce a detectable moiety as a product of that reaction or is a moiety capable of being cleaved from the tagged binding pair member itself, volatilized and detected. The methods and reagents herein described are particularly applicable in the analysis of physiological and biological samples derived from human as well as from animal, plant, and microorganismic sources. In addition, the methods and reagents may be employed in connection with environmental analyses, specifically, in assays for suspect chemical or biological constituents or contaminents in food, water, air, soil samples, and the like.</p> |
