| 摘要 |
Nucleic acid hybridization assays are performed by using probes that are immobilized on discrete, water suspensible microparticles (latex). Assay times under 30 minutes from the initiation of hybridization through measurement of the generated signal are obtained. The methods are based on detection of hybrids through the use of labeled anti-hybrid antibody reagents or by labeling the sample nucleic acids. The assay formats are convenient as well as rapid. The use of latex supports for the probe eliminates the problems of immobilizing sample nucleic acids, allows the use of excess probe, and enables rapid and simple separation of hybrids for detection purposes. |
