| 摘要 |
Fluorogenic compounds are disclosed which are reactive with biologically important analytes including primary amines, secondary amines, and/or thiols and are of the general formula: <IMAGE> wherein X is a group such as <IMAGE> -CN, -CO2CH3, or SO2R, and wherein R is <IMAGE> OR3, or Cl; R1 and R2 are hydrogen atoms or alkyl groups having 1 to 3 carbon atoms and R3 is a phenyl or benzyl group. The inventive compounds form a fluorescent adduct with the above analytes at the number 7 position of the benzoxadiazole ring. The fluorescent adduct thereby formed enables detection of very minute amounts of analyte (picomoles) using conventional fluorescent analytical methods.
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