Method of detecting and quantitating cell malignancy in biological tissue

摘要

A method of detecting and quantitating cell malignancy in biological tissue is provided and initially includes preparing a tissue sample having a substantially constant thickness. The tissue sample was impinged by filtered electromagnetic energy within an approximate bandwidth between 255.0-265.0 nm with a preferred wavelength of 260.0 nm. A standard Zeiss microscope was used to observe and measure the nuclear material with the microscope being fitted with quartz optics. The volume of nuclear material being examined was maintained relatively constant by selecting an aperture smaller in diameter than the magnified image of the nucleus being examined in combination with the subtantially uniform thickness of the tissue samples. The electromagnetic energy subsequent to being reflected from the tissue sample was passed through a second optical filter having an approximate filtering capacity in the range of 260.0 nm. The amount of electromagnetic energy within the predetermined bandwidth determined by the filters and absorbed by the nucleate of the cells within the tissue sample was measured. The amount of electromagnetic energy within the predetermined bandwidth absorbed by the nucleate was then quantified showing a higher absorptance of the filtered electromagnetic energy by malignant cells when taken with respect to normal cells.