| 摘要 |
<p>PURPOSE:To produce a lignan compound such as (+)-sesamin, (+)-sesamolin, etc., on an industrial scale in large quantity, by inducing a reproductive cell from a sesame plant and culturing the cell in a medium. CONSTITUTION:Slices of sprout, stalk, leaf and/or root of aseptically prepared seedling of sesame are cultured in a solid and/or liquid medium containing carbon source, nitrogen source, vitamins, nucleic acid relating substances, minerals, etc., and preferably added with phytohormones such as auxin, cytokinin, etc., in dark place at 28-30 deg.C and the induced reproductive callus obtained by this process is subcultured to form a large callus. The callus is grown in a medium containing naphthaleneacetic acid, benzyladenine, gelan gum, etc., and preferably added with natural organic nutrients in light place at 20-37 deg.C, preferably 25-35 deg.C and pH5.6-6.0. The objective lignan compound is extracted from the proliferated cell.</p> |
