| 摘要 |
PURPOSE:To industrially and advantageously obtain the titled fatty acid useful as perfumes, agents, paints, etc., by cultivating a specific strain in a culture medium containing a saturated fatty acid (derivative) or reacting resting microbial cells thereof with a saturated fatty acid (derivative). CONSTITUTION:A strain of the genus Rhodococcus separated from soil is irradiated with ultraviolet rays to provide Rhodococcus sp. KSM-B-3M which is a microorganism of the genus Rhodococcus, capable of producing an unsaturated fatty acid and exhibiting the following mycological properties. Shape; Bacilli having 0.5-0.8X1-5mum size. Growth range: 15-37 deg.C, pH5-9.5. Assimilating L-arabinose, etc. The resultant strain is then aerobically cultivated in a culture medium, such as glucose or peptone, containing 5-10wt.% saturated fatty acid (derivative) expressed by formula I [R is 9-21C straight-chain or branched alkyl; R1 is H or 1-10C straight-chain or branched (un)saturated hydrocarbon group, alkali metal or formula II (R2 and R3 are H or 1-5C alkyl group)] at pH6.5-8 and 25-35 deg.C for 1-3 days. The resultant culture is subsequently subjected to extraction and purification treatment to recover the aimed unsaturated fatty acid (derivative).
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