| 摘要 |
PURPOSE:To efficiently obtain the titled oligosaccharides useful as sweeteners, etc. by reacting a specific enzyme with a blend solution of starch or dextrin and N-acetylglucosamine, glucosamine, mannose or allose. CONSTITUTION:A microorganism, such as Bacillus subtilis, is inoculated into a culture medium containing polypeptone, etc. and aerobically cultivated at about 37 deg.C for 5-48hr to provide microbial cells, which are then subjected to extraction treatment to afford a disproportionating.enzyme (D-enzyme), reactive with maltooligosaccharides and exhibiting saccharide transfer action. The resultant enzyme is subsequently added to a solution obtained by mixing starch, such as corn, or dextrin with N-acetylglucosamine, glucosamine, mannose or allose at 1:1 ratio in 20-30wt.% starch concentration. The solution is then subjected to saccharide transfer reaction at about pH6.5 and about 35 deg.C for about 48hr. The reaction solution is subsequently subjected to filtration and concentration treatment to recover oligosaccharides containing the N- acetylglucosamine, glucosamine, mannose or allose at terminals.
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