METHOD FOR CONTROLLING EXPRESSION OF EXOGENOTE AND PRODUCTION OF EXOGENOTE PRODUCT USING SAID METHOD

摘要

PURPOSE:To readily and efficiently obtain an exogenote product useful in cultivation engineering field in a high concentration, by cultivating specific Escherichia coli while controlling saccharide concentration of a culture medium and controlling expression of the exogenote. CONSTITUTION:A recombinant plasmid pSYPL-3 prepared by inserting aL- phenylalanine.ammonia-lyase (PAL) structural gene into a vector containing a gene capable of coding ampicillin tolerance to provide an Escherichia coli MT 10424 strain capable of expressing PAL. The resultant strain is then inoculated into a culture medium containing glucose, etc., and kept at >=0.5wt.% concentration of saccharide utilized as a carbon source to carry out cultivation in the first process and multiply the amount of microbial cells. The saccharide concentration is subsequently kept at <0.5wt.% to carry out cultivation in the second process. Thereby control of exogenote expression is released to produce the aimed PAL which is an exogenote product in the culture medium.