| 摘要 |
PURPOSE:To portionwise sample a fractionated component at high purity efficiently and continuously without using an adsorbing column and without receiving the restriction of the number of times of fractionation, by providing a change-over valve between a separation column and a low pressure side and portionwise sampling the fractionated component by the operation of the change-over valve. CONSTITUTION:The first flow channel consisting of lines 9, 10 connects a change-over valve 2 and a separation column 1 and the second flow channel composed of a line 12 connects the change-over valve 2 and the separation column 1 through a minute flow rate control valve 7. The third flow channel consisting of lines 14, 15, 24 connects the change-over valve 2 and a low pressure region through a back pressure control valve 8, and the fourth flow channel consisting of lines 16-18, 20-22, 23 connects the change-over valve 2 and the low pressure region through a portionwise sampling change-over valve 3 and portionwise sampling containers a-c. When a supercritical fluid having a mixture to be separated dissolved therein is introduced into the separation column 1, the supercritical fluid wherein the mixture is fractionated into respective components is allowed to flow out from the separation column 1 in a time series manner. Hereupon, the change-over valves 2, 3 are operated in relation to a fractionated component detector 6 and the connection state of each flow channel is altered and each of the fractionated components is portionwise sampled in each of the portionwise sampling containers a-c. |
