Process of preparing single-stranded DNA.

摘要

Discolsed is a process of preparing a single-stranded DNA. In the first step of the process of the present invention, a single-stranded DNA containing a template DNA region is provided. Then a terminating structure which terminates the extension of DNA chain which is to be formed using the template DNA as a template in a later step is introduced at the 5' end portion of the template DNA. Then one or more primers are hybridized with one or more portion of the template DNA. Therafter, the primer is extended utilizing the template DNA as a template. Finally, the single-stranded DNA formed by the extension of the primer is separated and recovered.