| 摘要 |
PURPOSE:To produce recombinant human superoxide dismutase (SOD) containing theoretical amount of copper ion and having high enzyme activity, by treating a solution of apoenzyme containing no copper of recombinant human SOD or insufficient for the copper with a copper salt in the presence of beta- mercaptoethanol. CONSTITUTION:A microorganism transformed with recombinant DNA having a human superoxide dismutase (SOD) structural gene downstream plasma expressing regulatory gene is cultivated and induction synthesis of SOD is carried out to provide a humid germ cell, which is suspended in a proper water solution having 5-8pH, ground to afford supernatant. beta-Mercaptoethanol having 5-100mM concentration is added to the supernatant or supernatant, etc., obtained after treating precipitation of ammonium sulfate, and further a copper salt having anion of an organic or inorganic acid is added in a concentration of 0.15X10<-6>-15X10<-6>mol. based on 1g of the humid germ cell at 0 deg.C - room temperature and the mixture is allowed to stand or stirred at 0 deg.C - room temperature, preferably at low temperature of nearly 4 deg.C for 10min - 1 day to isolate the aimed SOD.
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