| 摘要 |
PURPOSE:To accurately select cell light information on desired cells without requiring complicated computation, by fractioning the cells according to inflection points of a histogram generated concerning parameters in the cell light information to select the cell light information. CONSTITUTION:A sample prepared is sent with a liquid feeder 3 into a flow cell 2 to be irradiated with a laser beam l and the intensity I0 of forward scattered light, the intensity I90 of 90 deg. scattered light, the intensity (Ig) of green fluorescence and the intensity (Ir) of red fluorescence are detected with photoelectric multipliers 7a-7d. An MPU 8 generates a histogram concerning the intensity I0 and the intensity I90 to extract inflection points thereof. According to the inflection points, a sightgram indicating the intensity I0 of forward scattered light on the axis of ordinate and the intensity I90 of 90 deg. scattered light on the axis of abscissa is divided into fractions I-IV. In the case of a lymphocyte subset analysis, light information on cells belonging to the fraction II is selected, fluorescence intensities Ig and Ir data are analyzed to generate histograms, which are displayed on a CRT 10. |
