摘要 |
PURPOSE:To obtain 3-chlorolactic acid useful as a synthetic raw material, etc., of useful compound in good yield, by reacting a bacterium cell of specific microorganism or treated product thereof with 3-chloro-1,2-propanediol. CONSTITUTION:A microorganism (e.g. Pseudomonas insueta ATCC21276) such as the genus Pseudomonas, Achromobacter, Candida, etc., having methanol- assimilating properties and ability capable of converting 3-chloro-1,2 propanediol (3CP) into 3-chlorolactic acid is aerobically cultivated in a culture medium containing a carbon source such as methanol, etc., nitrogen source, etc., such as urea, etc., at 4-8pH and 25-40 deg.C for 1-10 days. A bacterium cell separated from the resultant culture product is added to an aqueous solution having 1-100g/l concentration of 3CP and aerobically reacted at 20-70 deg.C and 5-11pH for 5-50hr to provide 3CL in the reaction liquid. Then the 3CL is recovered by solvent extraction method, ion exchange resin method, etc.
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