发明名称 |
High level expression in E. coli of soluble mature hIL-1beta and derivatives with altered biological activity. |
摘要 |
<p>Plasmid pUC8 and DNA coding for hIL-1 beta are used to construct hybrid plasmids capable of high level expression in E. coli of soluble proteins, including mature hIL-1 beta and derivatives of mature hIL-1 beta having amino acid substitutions and insertions at one or all of position 1 to 4 at the amino terminus. Derivatives of hIL-1 beta with alterations at the N-terminus have been produced which have either enhanced or decreased bioactivity compared to native monocyte derived hIL-1 beta .</p> |
申请公布号 |
EP0276778(A2) |
申请公布日期 |
1988.08.03 |
申请号 |
EP19880100924 |
申请日期 |
1988.01.22 |
申请人 |
E.I. DU PONT DE NEMOURS AND COMPANY |
发明人 |
HUANG, JAMES JEA-HUANG |
分类号 |
C12N15/00;C07K14/545;C12N15/72 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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