RECOMBINANT PLASMID DNAS, CODING FOR SYNTHESIS OF POLYPEPTIDE SUBSTANCES FOR A VACCINE AGAINST HEPATITIS A.

摘要

Recombinant plasmid DNAs, coding for synthesis of polypeptide substances for a vaccine against hepatitis A, consist of a fragment of the HAV genome and an expressing vector. As the fragment of the HAV genome are used: a fragment of the size 389 b.p., coding for the VP1 protein fragment from 4 to 129 a.a.; a fragment of the size 3372 b.p., coding for all structured VP4 proteins starting with 38 a.a., and for all non-structured HAV proteins; a fragment of the size 1243 b.p., coding for a polypeptide chain containing the VP3 protein fragments from 110 to 233 a.a. and the VP1 protein fragments from 1 to 271 a.a; a fragment of the size 144 b.p., coding for the VP1 protein from 4 to 57 a.a.; a synthetic fragment of DNA, coding for the VP1 protein fragment from 11 to 25 a.a. having the following structure: AATTCACAGTTAGTACTGAACAAAATGTACCAGATCCACAGGTAGGTATTGCGGTGTCAATCATGACTTG TTTTACATGGTCTAGGTGTCCATCCATA or a fragment of the size 4296 b.p. consisting of a fragment of the size 3372 b.p., coding for all the structured and some non-structured HAV proteins, and of a fragment of the size 924 b.p., coding for the viral protease. As the expressing vector are used: plasmid pUR 291, pUR 292, pIFN-fu1, pIFN-fu2 or plasmid pSPVV inserted in vaccinia virus LIVP as vector.