发明名称 |
ASSAY METHOD USING PARTICLES WITH ASSOCIATED LUMINESCER |
摘要 |
Assay methods are provided or determining an analyte in a sample suspected of containing the analyte. The method is carried out using a composition that includes a conjugate of a first sbp member with a particle. A luminescer is reversibly associated with a nonaqueous phase of the particle. Where the first spb member is not complementary to the analyte, a second sbp member that is capable of binding to the first sbp member is employed. Unbound conjugate is separated from conjugate that is bound to the analyte or to the second sbp member. A reagent for enhancing the detectability of the luminescer is added and the light emission of the luminescer acted on by the reagent is measured. |
申请公布号 |
EP0275139(A3) |
申请公布日期 |
1988.08.03 |
申请号 |
EP19880300033 |
申请日期 |
1988.01.05 |
申请人 |
SYNTEX (U.S.A.) INC. |
发明人 |
PEASE, JOHN;WENG, LITAI;KIRAKOSSIAN, HRAIR;ULLMAN, EDWIN F. |
分类号 |
G01N33/543;C09B23/01;C09B57/00;G01N33/544;G01N33/58;(IPC1-7):G01N33/58;G01N33/542;G01N33/537 |
主分类号 |
G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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