| 摘要 |
This invention relates to a method of detecting, analyzing, quantifying, or locating a substance of biological interest by an immunoassay in which a first component of an immune reaction in the form of an antigen, hapten, or antibody, is linked with one or more components of a chemiluminescent reaction, and the other component of the immune reaction, the analyte, is complexed therewith to cause entropic and/or ethalpic changes in a subsequent light emitting action as compared with the reaction of uncomplexed labelled component, the reaction being observed and compared to provide information on the immune complex formation. Preferably the intensity of light emission of the rate of photonic emission or the rate of change of intensity of emission are observed compared with the equivalent properties of the uncomplexed component.
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