HOMOGENEOUS HUMAN INTERLEUKIN 2 AND PROCESS THEREOF

摘要

RAN 4105/69 A process for preparing human interleukin 2 in homogeneous form which process comprises in combination passing a solution of crude human interleukin 2 through a reverse phase high performance column containing methyl or octyl groups covalently bonded to silica gel whereby said human interleukin 2 is retained by said column, eluting said column under HPLC conditions with a buffered n-propanol 0-60% (v/v) gradient and pooling fractions exhibiting human interleukin 2 activity; and passing the pooled active fractions from step A above through a reverse phase high performance column containing diphenyl groups covalently bonded to silica gel whereby said human interleukin 2 is retained by said column, eluting said column under HPLC conditions with a buffered n-propanol 20-60% (v/v) gradient and pooling fractions exhibiting human interleukin 2 activity and optionally repeating step A and/or B.