| 摘要 |
The reagents in the kit are included in an absorbant material, with the reagents in dry alternatively lyophilized form. The reagents are arranged in one of two ways. In Arrangement I the order of the reagents is (1) purified complexes of enzyme and antibodies against the antigen to be detected; (2) antigen immobilized in the absorbant material; and (3) substrate to the enzyme. In Arrangement II the order of reagents is (1) purified complexes of enzyme and antigen, which antigen is antigenically related to the antigen to be detected and which antigen is immobilized in the absorbant material; and (3) substrate to the enzyme. Arrangement I can also be used to detect antibody. In both cases, the test fluid is allowed to pass, by capillary forces, from step (1) to step (3). The presence of the antigen (antibody) to be tested for is thereby detected by the occurrence of a colour reaction at (3), due to the splitting of the substrate. The kit therefore allows a very simple procedure for detection of a compound without washing steps etc. usually included in the ELISA-technique. The invention also creates possibilities for quantitation by instrumental reading of the colour change at step (3). |
