| 摘要 |
A method is disclosed for determining the presence of a member of a specific binding pair ("sbp member") consisting of ligand and its homologous receptor in a sample suspected of containing the sbp member. The method comprises combining in an assay medium the sample and an opaque particle capable of agglutinating in the presence of the sbp member. The opaque particle has a particle size of from about 0.2 to 5.0 microns. Next, the assay medium is irradiated with light having a wavelength of from about 350 to 2000 nm, and the optical density of the assay medium is measured. A change in optical density indicates the presence of the sbp member in the sample. The method has particular application in the determination of an antibody in a sample, particularly an autoantibody, such as, for example, rheumatoid factor.
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