Purification of recombinant beta-interferon incorporating rp-hplc and formulations comprising the products thereof.

摘要

RP-HPLC methods for purifying recombinant beta-interferon are disclosed that employ wide-pore, silica gel reverse-phase high performance liquid chromatograph (RP-HPLC) columns and solvent systems containing acetonitrile as the organic modifier and either heptafluorobutyric acid or trifluoroacetic acid as the organic acid. The invention concerns processes for purifying recombinant IFN- beta incorporating this RP-HPLC method. The products may be formulated for pharmaceutical or veterinary use.