发明名称 |
Monoclonal antibodies with specificity for crosslinked fibrin derivatives and assay for said derivatives |
摘要 |
The invention relates to a method of a preparation of monoclonal antibody derived from a crosslinked fibrin derivative including the steps of: (i) obtaining a crosslinked fibrin derivative or extract containing same; and (ii) forming an antibody to said derivative or extract by cloning antibody producing cells from an animal having been administered thereto said derivative or extract. A screening assay for use in the abovementioned method which includes the steps of: (A) coating a surface with an antigen selected from a (i) crosslinked fibrin derivative; (ii) extract containing said derivative; or (iii) fibrinogen degradation product. (B) contacting said antigen with a monoclonal antibody derived from crosslinked fibrin derivative; and (C) subjecting the complex formed in step (A) to a signal amplification step and an assay procedure for detection of the presence of a crosslinked fibrin derivative in an animal body fluid including the steps of: (i) contacting a monoclonal antibody prepared from a crosslinked fibrin derivative with a fluid sample suspected of containing an antigen derived from a crosslinked fibrin derivative or comprising a crosslinked fibrin derivative per se; and (ii) subjecting the complex formed in step (i) to a signal amplification step are also provided.
|
申请公布号 |
US4758524(A) |
申请公布日期 |
1988.07.19 |
申请号 |
US19840590054 |
申请日期 |
1984.03.15 |
申请人 |
FIELDER GILLESPIE DAVID LIMITED |
发明人 |
BUNDESEN, PETER G.;RYLATT, DENNIS B. |
分类号 |
C12N15/02;A61K39/395;C07K16/18;C12N15/00;C12P21/00;C12P21/08;C12Q1/00;C12R1/91;G01N33/53;G01N33/577;G01N33/86;(IPC1-7):G01N33/53 |
主分类号 |
C12N15/02 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|