摘要 |
An embryo freezer is disclosed in which the freezing chamber is mounted in the neck of a liquid nitrogen tank. The rate of cooling of the freezing chamber is regulated by adjusting the height of the freezing chamber within the neck of the liquid nitrogen tank. The embryo is stored in a straw which is in turn suspended in liquid methyl hydrate within the freezing chamber. Air is bubbled through the liquid in the freezing chamber to maintain a uniform temperature throughout the freezing chamber.
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