| 摘要 |
<p>PURPOSE:To supply a culture liquid with O2 in high efficiency without causing foaming of the liquid and preventing the damage of cells caused by shearing force and to culture animal cells in the liquid, by mechanically stirring a culture liquid in a culture tank suppressing the disturbance of the liquid surface and the formation of vortex and continuously feeding an O2-containing gas to the liquid surface under a specific condition. CONSTITUTION:A mechanical stirring culture tank 1 is placed in a thermostatic bath 5. A stirring blade 3 having a shape to suppress the disturbance of the liquid surface and the formation of vortex is placed in the culture liquid in a manner to be rotated with a stirring motor 4. A gas-feeding pipe 2 composed of a buffer pipe 2a, a blasting nozzle 2b and an aeration pipe 2c is attached to the space above the liquid. The culture liquid in the culture tank 1 is stirred and, at the same time, an O2-containing gas is supplied through an aeration port 11 to the gas-feeding pipe 2 in a manner that the product of the feeding rate and the linear velocity (5-65m/sec) of the gas at the blasting end of the nozzle 2b falls within the range of 1.0X10<3>Xh-2.5X10<5>Xh (h is distance between the tip of the nozzle 2b and stationary liquid surface). The gas is continuously blasted from the nozzle 2b against the liquid surface to form a hollow part on the surface. Animal cells are cultured in the culture liquid under the above condition.</p> |
