摘要 |
<p>PURPOSE:To prepare a high-quality brewed liquor having excellent storage stability, by contacting brewed liquor with an immobilized protease after filtering the liquor to remove dregs from the liquor, thereby inactivating the enzyme in a short time without deteriorating the excellent flavor and taste of refined liquor. CONSTITUTION:One or more proteases selected from pepsin, trypsin, papain and heat-resistant protease are immobilized on a polymeric insoluble carrier having nonionic hydrophilic functional groups on the surface e.g. by physical adsorption process and the carrier supporting the enzyme is packed in a small- capacity column. A brewed liquor finished with brewing step and filtration step for the removal of dregs and, if necessary, with aseptic treatment step with a filter for precise filtration, aseptic and deproteinizing step by ultrafiltration or sterilization step with ultraviolet ray is passed through the column and made to contact with the carrier for a short time.</p> |