| 摘要 |
The invention pertains to a method for simultaneous real time fluorometric sequence analysis of nucleic acid fragments of different lengths and four different terminals, the fragments being labelled with different fluorescent labels for the respective terminating bases. The fragments are labelled with four different labels comprising the same two lanthanides and the same two ligands as follows: label 1 = ligand 1 + lanthanide 1, label 2 = ligand 2 + lanthanide 1, label 3 = ligand 1 + lanthanide 2, label 4 = ligand 2 + lanthanide 2, where ligands 1 and 2 have different excitation wavelengths and lanthanides 1 and 2 have different emission wavelengths, excitation light is generated in the form of light pulses alternating between the different excitation wavelengths of the ligands, and the photons emitted by the labels are detected periodically for the different emission wavelengths of the lanthanides. |
