发明名称 |
DNA VECTORS AND THEIR USE IN RECOMBINANT DNA TECHNOLOGY |
摘要 |
The invention provides a new class of DNA vectors, each comprising two replication systems; a first origin of replication resulting in a low copy number and stable inheritance of the plasmid, and a second, high copy number, origin of replication at which replication is directly controllable such that, when host cells carrying the vector are propagated under a first set of conditions, replication takes place mainly from the low copy number origin, and that when said cells are propagated under a second set of conditions, replication takes place also from the high copy number origin to produce a high yield of gene product. The controllable origin of replication may be under the control of a natural promoter of RNA transcription or a substitute promoter such as the PL promoter or lac promoter. |
申请公布号 |
DE3471688(D1) |
申请公布日期 |
1988.07.07 |
申请号 |
DE19843471688 |
申请日期 |
1984.03.23 |
申请人 |
CELLTECH LIMITED |
发明人 |
YARRANTON, GEOFFREY THOMAS;HUMPHREYS, GWYNFOR OWEN;ROBINSON, MARTYN KIM;CAULCOTT, CELIA ANN;WRIGHT, EDWINA MARY |
分类号 |
C12N9/52;C07H;C12N;C12N9/10;C12N9/64;C12N15/00;C12N15/09;C12N15/67;C12N15/69;C12N15/70;C12P;C12R1/19;(IPC1-7):C12N15/00;C12N9/50 |
主分类号 |
C12N9/52 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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