发明名称 |
RECOMBINANT DNA EXPRESSION VECTORS AND DNA COMPOUNDS |
摘要 |
The present invention comprises novel DNA compounds that encode isopenicillin N synthetase and also comprises related methods, transformants, and polypeptides. The novel isopenicillin N synthetase-encoding DNA, together with its associated transcriptional and translational activating sequence, was isolated from Cephalosporium acremonium and cloned into an E. coli cloning vector. The isopenicillin N synthetase-encoding DNA has been used to construct novel E. coli expression vectors that drive expression of a stable, active, and novel isopenicillin N synthetase in E. coli. The intact C. acremonium isopenicillin N synthetase-encoding DNA and associated transcriptional and translational activating sequence have also been used to construct C. acremonium expression vectors that drive expression of the isopenicillin N synthetase in C. acremonium. The C. acremonium transcriptional and translational activating sequence has further been fused to a hygromycin phosphotransferase-encoding DNA segment and placed onto C. acremonium expression vectors. Useful derivatives of the novel compounds and vectors are also described. |
申请公布号 |
EP0200425(A3) |
申请公布日期 |
1988.07.06 |
申请号 |
EP19860302873 |
申请日期 |
1986.04.17 |
申请人 |
ELI LILLY AND COMPANY |
发明人 |
INGOLIA, THOMAS DOMINICK;QUEENER, STEPHEN WYATT;SAMSON, SUELLEN MARY;SKATRUD, PAUL LUTHER |
分类号 |
C12N15/52;C07H21/04;C12N1/00;C12N1/21;C12N9/00;C12N15/00;C12N15/09;C12N15/69;C12N15/70;C12N15/74;C12N15/80;C12P37/00;C12R1/01;C12R1/19;C12R1/365;C12R1/465;C12R1/75;C12R1/82 |
主分类号 |
C12N15/52 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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