| 摘要 |
PURPOSE:To easily obtain an immobilized enzyme having high activity and stability, by impregnating an enzyme-containing water-soluble polymer substance in a carrier, removing water from the polymer and forming a thin film or polymer on the surface of the carrier. CONSTITUTION:An aqueous solution of a water-soluble polymer substance such as polysaccharides, proteins, having a concentration of 0.1-5wt% and containing 1-10mg/l of an enzyme such as oxidoreductase, transferase, hydrolase, isomerase, detaching enzyme, synthase, is prepared beforehand. The aqueous solution is impregnated in a preparatorily washed and dried carrier such as activated carbon, cellite, porous glass, natural fiber, water is removed e.g. by frreze-drying and the product is made to contact with an organic solvent solu tion of a film-forming synthetic resin having a number-average molecular weight of <=100,000 and soluble in an organic solvent. The solvent is evaporated to form a thin film on the surface of the carrier and immobilize the enzyme.
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