| 摘要 |
1,219,147. Chromatographic separation of amino add mixtures. CESKOSLOVENSKA AKADEMIE VED. 19 April, 1968 [25 April, 1967], No. 18665/68. Heading B1X. Amino acid mixtures which include tyrosine and phenylalanine are eluted after separation on a chromatographic column, using initially as eluent for the acidic and neutral amino acids a 0.2N citrate buffer of pH 4.25 until valine has been eluted, and thereafter, to separate the tyrosine-phenylalanine pair, a 0.38N buffer of pH 5.28. To assist the separation of the threonine-serine pair, the column is preferably first stabilized with a citrate buffer containing a small proportion of an organic solvent, suitably a lower alcohol such as methanol or n-butanol. A suitable schematic arrangement in which the sequential steps of the separation may be performed is also described (see Fig. 2, not shown) the fractions being photometrically detected after reaction with ninhydrin in a capillary reactor. |
