Multilayer analysis elements for the determination of total cholesterol.

摘要

A multilayer analysis element for determination of total cholesterol comprising a light-transmissive water-impermeable support, at least one hydrophilic polymer layer on said support and a spreading layer on said hydrophilic polymer layer(s), and containing a cholesterol analysis color reagent composition comprising: (a) at least one enzyme having cholesterol estrase activity, (b) cholesterol oxidase, (c) peroxidase, and (d) a color reagent composition; wherein said element further contains: (e) at least one bile acid compound selected from the group consisting of bile acids, bile acid derivatives and sales of said acids and derivatives, and (f) an alkyl phenoxy polyethoxy ethanol containing a polyoxyethylene chain composed of at least 16 oxyethylene units.