Method for amplification and detection of RNA sequences.

摘要

A process for the rapid amplification and detection of a RNA sequence which comprises adding to RNA two converging oligodeoxyribonucleotide primers at least one of which is complementary to a sequence included in said RNA; thereafter extending at least one of said primers with one or both of reverse transcriptase and DNA polymerase I, determining whether said sequence is present in said amplified segment by use of synthetic oligodeoxyribonucleotide probe complementary to a region within the segment.