发明名称 |
Signal enhancement in immunoassay by inhibition of enzymatic catalysis. |
摘要 |
<p>A method for enzyme immunoassay for a ligand suspected to be present in a liquid sample includes signal amplification by use of at least two enzymes and a blocked modulator for one of the enzymes. Ligand present in the liquid binds to an antiligand and an enzyme-labeled tracer. The resulting bound fraction is separated and the enzyme in the tracer removes the blocking group from the blocked modulator. The modulator activates or inhibits a second enzyme which catalyzes the conversion of a substrate to a product. The presence or absence of the ligand in the liquid is indicated by a signal, such as a color change or a rate of color change, associated with the product. The invention includes a new class of enzyme inhibitors and blocked inhibitors and a kit of materials useful for performing the method of the invention.</p> |
申请公布号 |
EP0271731(A2) |
申请公布日期 |
1988.06.22 |
申请号 |
EP19870117014 |
申请日期 |
1987.11.18 |
申请人 |
BECTON, DICKINSON AND COMPANY |
发明人 |
MIZE, PATRICK D.;O'CONNELL, JAMES P. |
分类号 |
G01N33/543;C07C45/51;C07C45/52;C07C45/63;C07C45/67;C07C49/16;C07C49/173;C07C49/245;C07C215/40;C07C225/06;C07F9/09;C07F9/12;C12Q1/00;C12Q1/37;C12Q1/42;C12Q1/44;C12Q1/46;G01N33/542;G01N33/58 |
主分类号 |
G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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