| 摘要 |
PURPOSE:To purify proteins in a short time without necessitating dialysis, dilution at a high ratio or decomposition with protease, by subjecting a soluble foreign protein to hydrophobic chromatography and metal chelate chromatography. CONSTITUTION:A soluble foreign protein produced by a microbial host such as E.coli by genetic engineering technique and different from the protein of the host (e.g. human plasminogen activator produced by E.coli) can be purified in high efficiency by treating the protein with a hydrophobic chromatography using a column packed with particles of phenylated agarose gel, etc., and a metal chelate chromatography using a column packed with particles of biscarboxymethylated agarose, etc. Preferably, the foreign protein is folded prior to the chromatographic treatment.
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