| 摘要 |
PURPOSE:To effectively remove remaining free radicals by removing a basic radical accelerating agent added to a two-dimensional gel by pre-migration and adding a strong acidic soln. to a negative electrode buffer at the time of analyzing a protein sample by using a bar-shaped gel, one-dimensional gel and two-dimensional gel consisting of polyacrylamide. CONSTITUTION:The sample is subjected to electrophoresis in the bar-shaped gel for a pretreatment and is concd. to prepare a zero-dimension sample gel. This gel is inserted at a desired level position in the one-dimensional gel and is subjected to one-dimensional electrophoresis. The gel subjected to the one- dimensional sepn. to be plated on the top end of the two-dimensional gel and to be subjected to electrophoresis is thereby prepd. The sample is concd. to assure the effective one-dimensional and further two-dimensional migration so as to avoid encountering of the individual protein molecules in the sample with free radicals by the zero-dimensional migration. The radical capturing agent is migrated in the respective stages and the strong acidic soln. is added to the negative electrode buffer to increase the acidity of the gel. |
