| 摘要 |
<p>PURPOSE:To obtain the titled compound useful as a remedy for skin diseases efficiently, by transforming a cell of a plant of the family Boraginaceae with plasmid of Agrobacterium rhizogenes, cultivating a formed hairy root in a medium containing nitrate ion, etc., and taking out a culture product after the cultivation. CONSTITUTION:A seed of a plant of the family Boraginaceae is sterilized with a fungicide such as a solution of sodium hypochlorite, etc., the seed is put on a solid medium, a cell of stem, leaf, etc., of germinated sterilized plant is inoculated with Agrobacterium rhizogenes containing Ri plasmid and transformed. Then after two-five weeks, a hairy root growing from the inoculated site is cut out, cultivated in a liquid medium containing 50-80mM nitrate ion and/or 0.00015-0.0003mM copper ion in 1l liquid medium and a substance produced from the hairy root is taken out to give a naphthoquinone compound such as shikonin shown by the formula (R is OH or OCOCH3), etc.</p> |
