| 摘要 |
PURPOSE:To produce hyaluronic acid having high molecular weight and useful as cosmetic and pharmaceutical, preventing the molecular weight lowering caused by the decomposition of hyaluronic acid, by adding a protein denaturation agent during or after the culture of microorganism capable of producing hyaluronic acid. CONSTITUTION:A microbial strain capable of producing hyaluronic acid is cultured in a medium containing carbon sources, inorganic salts, organic nutrients, etc., at about 30-35 deg.C under aeration and agitation. At an arbitrary stage of or after the culture, the system is added with a protein denaturation agent to obtain hyaluronic acid having high molecular weight. The microbial strain capable of producing hyaluronic acid is e.g. Streptococcus zooepidemicus, Streptococcus equisimilis, etc., and the protein denaturation agent is preferably protease, guanidine hydrochloride, sodium laurylsulfate, etc. the amount of the denaturation agent is preferably about 0.005-1.0% for sodium laurylsulfate.
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