| 摘要 |
<p>The invention relates to methods for medicinal diagnosis and to substance for obtaining such diagnoses. More specifically the substances are visualization polymers of molecules such as proteins, enzymes, and chemically tagged polyols, polyolefins, carbohydrates and natural or synthetic polypeptides, which can be combined with biological material and provide substantial chemical amplification of the quantity of material detected. The specification discloses a method for visualizing the presence of an inorganic or organic target molecule in a biological material, which comprises: combining said target with a visualization polymer comprising multiple units of a visualization monomer directly bonded together or linked by a coupling agent through chemical groups or backbone moieties of said units; said visualization monomer having at least one visualization site and being selected from an enzyme, a tagged natural or synthetic polypeptide, a tagged polyol, a tagged polyolefin or a tagged carbohydrate; said chemical group being a amine group, an oxidized 1,2-diol group, a carboxy group, a mercaptan group, an hydroxy group or a carbon-hydrogen bond; said backbone moiety being an amide bond, a carbon-carbon bond, a carbon-oxygen bond, or a carbonhydrogen bond; and said chemical group or backbone moiety being located within said monomer at a position which is at least one atom away from the visualization site of said monomer.</p> |
