NOVEL HYDROLASE AND METHOD

摘要

A substantially enzymatically pure hydrolase is provided which is secreted by and isolatable from Pseudomonas putida ATCC 52552. Cloning the gene expressing the hydrolase into a suitable expression vector and culturing, such as fermenting the E. coli strain JM101 harboring a plasmid designated pSNtacII, has been found to provide surprisingly high yields of the hydrolase.