FREMGANGSMAATE FOR MAGNETISK SEPARERING AV MERKET REAGENS I EN IMMUNOMETRISK BINDINGS-ANALYSE.

摘要

A method for the magnetic separation of the free species of a labeled reagent in an immunometric binding assay involving binding reactions in a liquid reaction mixture among analyte from a liquid test medium, a magnetically responsive reagent particle comprising the analyte or a binding analog thereof immobilized thereto, and a labeled reagent comprising a specific binding partner for the analyte labeled with a detectable chemical group. Analyte from the test medium binds to the labeled reagent and any of the labeled reagent not so bound binds to the magnetically responsive reagent particle which are separated on a chromatographic separation and detection test device comprising a separation zone and a detection zone for receiving the analyte bound to the labeled reagent. The reaction mixture and a magnetic field are applied to the separation zone whereby the magnetically responsive reagent particle is magnetically retained at the separation zone and the analyte bound to the labeled reagent is transported into the detection zone where the detectable chemical group thereof provides a detectable signal which is measured and correlated to the amount of analyte in the liquid test medium.