| 摘要 |
PURPOSE:To obtain a foreign protein having high activity, by adding a heat- treatment step to the denaturation and solubilization of a foreign protein with a protein-denaturation agent and applying the process to the treatment of a foreign protein such as urokinase produced e.g. by recombinant DNA technique. CONSTITUTION:A foreign protein such as pro-type urokinase granule is denaturated and solubilized with a protein-denaturation agent (e.g. guanidine hydrochloride). The foreign protein can be activated by adding a heat-treatment step to the above process. Concretely, a foreign protein is denaturated and solubilized with a protein-denaturation agent and heat-treated in the protein- denaturation agent at 30-90 deg.C for 40-90min. The granules which are left insoluble and proteins denaturated and insolubilized by the heat-treatment are removed by centrifugal separation and the supernatant liquid is diluted with a solution containing glutathione, EDTA, etc., to obtain a reactivated foreign protein.
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