摘要 |
PURPOSE:To obtain an immobilizing carrier, consisting of DVB polymer particles having a macroporous structure and capable of readily and efficiently carrying out immobilization of enzymes, etc., for reaction of a water-insoluble substrate. CONSTITUTION:50-120pts.wt. precipitating agent, preferably 4-methyl-2-pentanol is added to 100pts.wt. high-purity DVB (divinylbenzene) or industrial DVB monomer consisting of a blend of ethylvinylbenzene with other various kinds of saturated compounds and suspension polymerization is carried out in water. The resultant polymer particles are initially washed with water, then methanol and further extracted with acetone until a constant weight is attained in a Soxhlet extractor. The precipitating agent and unreacted monomer remaining in the particles are removed to afford the aimed macroporous DVB polymer particles having 0.25-1.65cc/g pore volume (measured by a mercury porosimter) and a pore diameter within the range of 140-2,000Angstrom . The above-mentioned particles are directly used as a carrier for immobilization without requiring activation treatment.
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