| 摘要 |
NEW MATERIAL:The cultured and established human cell [AGR-ON] originated from human leukemia T cell and having the following characteristics. (1) Appearance, lymphoid cell; (2) proliferation property, highly proliferative in RPMI- 1640 medium containing 100muM of 8-azaguanine, 10% bovine fetus serum, 5X<-5> M of 2-mercaptoethanol, 100mug/ml of streptomycin, 100 unit/ml of penicillin G, 50mug/ml of gentamycin, and 1mM of glutamine; (3) subculture, able to continue without limitation; (4) freeze-storage, storable for a long period in liquid nitrogen; (5) enzyme deficiency, it is an HGPRT enzyme-deficient strain and is exterminated in an RPMI-1640 medium containing 1X10<-4>M of hypoxanthine, etc.; (6) number of chromosomes, 43-54; (7) substance-producing capability, continuously produces a colony-formation stimulating factor (CSF). USE:Useful for the preparation of CSF (a remedy for cancer, infectious diseases, etc.). PREPARATION:The objective cell can be prepared by the cultivation of human leukemia T cell strain. |
