DIAGNOSIS OF NEPHROPATHY BY MEASUREMENT OF RENAL ANTIGEN IN URINE USING MONOCLONAL ANTIBODY

摘要

PURPOSE:To measure an amount of antigen discharged in urine and to specify a disorder site, by preparing a monoclonal antibody to be specifically reacted with a human normal renal tissue antigen such as human lumen wall of renal uriniferous tubule, basement membrane of glomerulus, etc. CONSTITUTION:An antibody-forming cell of an animal immunized against a normal human renal tissue or Hela cell is fused with a myeloma cell to give a hybridoma, which is cultivated to produce a normal human renal tissue. A monoclonal antibody to be specifically reacted with the normal human renal tissue is recovered to produce a monoclonal antibody. A disorder site in the kidney and the degree of the disorder are diagnosed by measuring an antigen in urine by using the monoclonal antibody to specifically recognize the human normal renal tissue antigen. The diagnose is carried out by comparing a measured value of antigen in specimen urine with a value of antigen in normal human urine.