| 摘要 |
Production of a desired heterologous protein comprises constructing an expression vector having a stabilizing sequence downstream of a promoter and upstream of the DNA encoding the desired heterologous protein, transfecting and choosing a particular eukaryotic host cell for said production and culturing the transformed eukaryotic host cell under favorable conditions. The stabilising sequence is an intron of a donor-acceptor-splice sequence, preferably from the immediate early gene of human cytomegalovirus or an immuloglobulin variable region. Vectors can be used for continuous production of factor VIII, t.PA and prorelaxin.
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