发明名称 Cloning vectors for expression of exogenous protein
摘要 A recombinant DNA cloning vector useful for expressing exogenous protein is described, which comprises (a) a DNA segment containing a functional origin of replication; (b) one or more DNA segments, each of which conveys to a transformable host cell a property useful for selection when said vector is transformed into said host cell; and (c) a DNA segment comprising a sequence that defines, in tandem, (1) the promoter of a lipoprotein expression control sequence, (2) the 5' untranslated region of a lipoprotein expression control sequence and (3) a translation start codon followed, without interposition of a portion or all of a nucleotide sequence coding for endogenous protein, by a nucleotide sequence coding for an enterokinase cleavage site to which is joined, without interruption, a nucleotide sequence coding for an exogenous protein.
申请公布号 US4745069(A) 申请公布日期 1988.05.17
申请号 US19840586581 申请日期 1984.03.06
申请人 ELI LILLY AND COMPANY 发明人 MAYNE, NANCY G.;BURNETT, J. PAUL;BELEGAJE, RAMAMOORTHY;HSIUNG, HANSEN M.
分类号 C12N15/66;C12N15/70;(IPC1-7):C12N15/00;C12N1/20;C12P21/00;C12P19/34;C07H21/02 主分类号 C12N15/66
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