| 摘要 |
PURPOSE:To improve measurement accuracy by irradiating a reaction system and a comparison system impulsively by a light source from outside a thermostatic chamber, finding the heat of reaction of the reaction system from the temperature difference between both systems, and calculating an oxygen reaction rate from it. CONSTITUTION:The light source part 3 is made to emit pulse light as shown by the upper stage (a) (lateral axis: time (t), longitudinal axis: heating value Q) of a figure, and thus the reaction system 1 and comparison system 2 in a balanced state at constant temperature are supplied with heat energy. Consequently, light energy absorption plates 10 of the reaction system 1 and comparison system 2 rise in temperature, but both systems differ in temperature variation state owing to a difference between the presence and absence of enzyme as shown by the intermediate stage (b) and lower stage (c) (lateral axis: time (t), longitudinal axis: temperature theta, temperature difference: DELTAQ) of the figure. For the purpose, the temperature variation between both systems after the pulse light emission is measured to measure the enzyme reaction rate, thereby knowing the enzyme concentration.
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